Fig. 4. Mislocalisation of mid-zone antigens in telophase cells of asp^dd4 mutants. Localisation of actin (A), Pav-KLP (B) and Peanut (C) are shown in red in the merged image and in monochrome in the central panels. The DNA is blue and the microtubules are green. The corresponding images of microtubules are also shown in monochrome in the bottom panels. In each panel the inset shows the localisation of the corresponding proteins at an equivalent stage of wild-type meiosis. (A) Actin is not organised into a contractile ring as in the wild type but is dispersed in the central part of the cell. One exceptional cell in which the actin is sufficiently well organised to cause constriction of the cell is seen in the very bottom left of this field. Pav-KLP is present in ring-like structures in some cells (large arrow), but in the majority, it has a more diffuse distribution in the central part of the cell (the small arrow indicates one such example). It does stain distinct ring canals (arrowheads), indicating that cytokinesis has occurred in earlier division cycles. (C) In contrast to the wild-type telophase cell, Peanut does not accumulate in a contracted ring-like structure but remains in a wide band around the circumference of the mutant telophase cell. In some cells (arrow), the central spindle is better organised than others (arrowhead).