Fig. 1. Colocalization and co-immunoprecipitation of endogenous Ce-emerin and Ce-lamin in wild-type C. elegans embryos. (A) Panels show indirect immunofluorescence staining of wild-type C. elegans embryos with antibodies against endogenous Ce-emerin (red; serum 3272), endogenous Ce-lamin (green), and the overlap of both signals (yellow). Bar, 10 µm. (B) Ce-emerin and Ce-lamin are co-immunoprecipitated from C. elegans embryonic lysates by immune (I) but not preimmune (P) antibodies raised against an N-terminal peptide (serum 3930) or a C-terminal peptide (serum 3932) of Ce-lamin. Shown is a western blot of immunoprecipitates probed with antibodies against Ce-emerin (see Materials and Methods). X indicates a blank lane.