Fig. 5. Nuclear envelope localization of Ce-emerin depends on Ce-lamin. Ce-lamin-deficient (lmn-1(RNAi)) embryos were stained for DNA and double-stained by indirect immunofluorescence for endogenous Ce-lamin and Ce-emerin. (Top panels) Triple staining of an embryo depleted of Ce-lamin by injecting lmn-1 dsRNA (`injection'). (Bottom panels) Triple-stained embryos were inefficiently depleted of Ce-lamin by the `feeding' method (see Materials and Methods), producing nuclei with nearly normal amounts of Ce-lamin (arrow), or reduced levels of Celamin plus mildly reduced (barred arrowhead) or severely reduced (arrowhead) levels of Ce-emerin at the nuclear envelope. Nuclear envelope rim-staining for Ce-emerin decreased in parallel with Ce-lamin. Bars, 10 µm.