Fig. 3. Kinetochore staining of wild-type and klp cells. Mis12-GFP (Goshima et al., 1999) marked the kinetochores (green) and Hoechst 33342 stained the DNA (blue); the direction of subsequent kinetochore movement is indicated by the yellow arrows. (A-D) Wild-type cells with the expected clustered kinetochores throughout mitosis. (E-H) klp5 cells with the sister kinetochores on each chromosome separated by 0.6 µm, and moving back and forth along the spindle. Bar, 5 µm.