Fig. 4. Deletion of klp5⁺ or klp6⁺ extends the time in mitosis, and missegregating chromosomes appear early in mitosis. (A) Graph of the total fraction of klp5 cells in mitosis (total mitotic) and the fraction of those cells with wild-type (mitotic-NORMAL) versus abnormal (mitotic-ABNORMAL) DNA segregation. The lines show a decay in the frequency of abnormal cells with a corresponding increase in normal cells (r²=0.97). (B) The frequency of the appearance of segregating DNA in cdc25-22 and klp5 cdc25-22 strains after cell cycle arrest and release. The fraction of cells in mitosis shows a Gaussian distribution (r²=0.98) for both strains. The area under the curve for klp5 cdc25-22 is 52% greater than that for cdc25-22 cells and its mode is shifted to the right by 22%. Approximately 400 cells were counted for each time point.