Fig. 5. Klp5p and Klp6p localize to kinetochores before anaphase. Klp5-GFP and Klp6-GFP were visualized in live cells (green) with Hoechst 33342 staining DNA (blue). (A-D) Klp5p-GFP in different cells through mitosis: prometaphase (A); metaphase (B); anaphase A (C); and anaphase B (D) (t=0-27.4 minutes). Klp6p-GFP in the same cell as it proceeds through mitosis. (E) Klp6p-GFP in the same cell as it proceeds through mitosis. Bar, 5 µm.