Fig. 5. Perinuclear distribution of huntingtin. 3T3 cells were stained simultaneously with the anti-huntingtin (green) and the anti-ß-tubulin (red) antibodies used in Fig. 4. Thin sections of the cells were then examined by confocal microscopy. In these little-exposed photomicrographs, huntingtin staining is mostly perinuclear, where it overlaps with the denser part of the microtubular network.