Fig. 4. ida mutants display abnormal chromosome cytology. Mitotic figures of wild-type prometaphase (A), wild-type anaphase (B), ida¹⁰⁷ prometaphase (C), and ida¹⁰⁷ anaphase (D) are presented. Prometaphase figures show that ida cells contain an increased number of chromatin structures (compare A with C). Aberrant anaphase figures with lagging chromosomes are observed in ida cells (arrow, D). Wild-type (E) and ida¹⁰⁷ (F) cells show normal staining patterns for tubulin (white) and centrosomin (green). Hoechst staining (blue) shows the position of chromosomes along the spindle.