Fig. 6. Bub1 is detected at ida kinetochores throughout mitosis. Hoechst staining (A-C) shows chromosome morphology and Bub1 staining (D-F) marks kinetochore positions. In all panels, asterisks mark prometaphase, arrows mark metaphase, and brackets mark anaphase. Two fields of ida¹⁰⁷ cells are shown (B,C,E,F). Bub1 staining is present on prometaphase figures from both wild-type (D) and ida¹⁰⁷ cells (E,F). During anaphase, Bub1 staining decreases in wild-type (bracket, D). The staining intensity is the same at both stages in ida¹⁰⁷ cells (brackets, E,F). Note that some ida mutant cells are outside the plane of focus.