Fig. 1. Integrin LFA-1 has higher affinity for lipid raft patches on J-ß2.7 cells expressing I-LFA-1 than on J-ß2.7 cells expressing wt LFA-1. Cells were incubated with TRITC-conjugated Ctx-B, then crosslinked with rabbit anti-Ctx-B antibody. Cells were fixed and stained with mAbs against LFA-1 (A), DAF (B), or TfR (C), followed by Alexa 488-conjugated goat anti-mouse IgG. Single optical sections taken at mid-height of the cells are shown. Top panels, I-LFA-1-expressing cells; bottom panels, wt LFA-1-expressing cells. Cell surface proteins, green; Ctx-B, red. Data are representative of six experiments (A), and three experiments (B,C). Bars, 10 µm.