(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. Depletion of cellular cholesterol by MßCD reduces clustering of {alpha}4ß1 on the surface of J-ß2.7 cells expressing {Delta}I-LFA-1 but has no effect on {alpha}4ß1 on wt LFA-1-expressing cells. Cells with (B,D) or without (A,C) pretreatment with 10 mM MßCD were stained on ice with the anti-{alpha}4 mAb 7.2R, fixed and incubated with Alexa 488-conjugated goat anti-mouse IgG, followed by confocal microscopy. (A,B) J-ß2.7 cells expressing {Delta}I-LFA-1. (C,D) J-ß2.7 cells expressing wt LFA-1. Single optical sections taken at mid-height of the cells are shown. Data are representative of two experiments. Bar, 10 µm.