Fig. 7. Cytochalasin D treatment affects colocalisation with lipid raft patches of LFA-1, 4ß1 and DAF, but has no effect on the distribution of TfR. Cells were preincubated with 5 µM cytochalasin D, then subjected to lipid raft patching and cell surface protein staining, as in Fig. 1: LFA-1 (A); 4ß1 (B); DAF (C); TfR (D). For each A-D section, top panels, I-LFA-1-expressing cells; bottom panels, wt LFA-1-expressing cells. Cell surface proteins, green; Ctx-B, red. Single optical sections taken at mid-height of the cells are shown. Data are representative of three experiments. Bar, 10 µm.