Fig. 4. Overexpression of NtKIS1a decreases organ size in increasing cell size. (A) A photograph of flowers from an extreme 35S::NtKIS1a line (NtKIS1a) and from wildtype (WT) indicating the three regions (I, II, III) of the petal that were used for microscopic analysis. (B) Light microscopic analysis of outer epidermal cells at three positions (I, II and III) of 35S::NtKIS1a and WT petal. (C) Cell size in the three regions of 35S::NtKIS1a and WT petals. Bars indicate s.d. (D) Scanning electron microscopy of the leaf lower epidermis of WT and two 35S::NtKIS1a Arabidopsis lines, one medium (M) and one strong (S). Bars, 100 µm. (E) From several scanning electron microscopy images of the lines described in D, cell areas were measured using the Optimas 6.0 software. The mean cell areas were reported in three categories: areas less than 300 µm², areas between 300 and 3000 µm² and areas greater than 3000 µm² (n.o. correspond to categories not observed). The number of cells (n) recorded in each categories is indicated in brackets.