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Fig. 5. Overexpression of NtKIS1a inhibits rosette leaf cell endoreduplication. Ploidy distributions for nuclei isolated from old (RL2) and young (RL1) rosette leaves, cauline leaves (CL) and flower buds (FB) of A. thaliana wildtype (WT), strong 35S::NtKIS1a (NtKIS1a) or 35S::NtKIS1b (NtKIS1b) lines. For each sample, 5000 DAPI-stained nuclei were analysed. The x-axis represents DNA fluorescence in logarithmic scale and DNA content is indicated. The y-axis represents frequency.