Fig. 1. Differential tranlsocation of PKC isoenzymes during cell cycle. U937 cells were synchronised by centrifugal elutriation and proteins were extracted from the cytosol (C) or particulate/membrane (M) fraction of whole cells in G₁, S or G₂/M. Extracts were analysed by western blotting and probed for the presence of PKC isoenzymes as indicated. The location of an 84 kDa marker protein is shown to the left of the figure. The data shown are representative of four separate experiments.