Fig. 2. Nuclear translocation of PKC-ßII during G₂/M. U937 cells were synchronised by centrifugal elutriation. (A) Proteins were extracted from nuclei of unfractionated cells (U) or cells in G₁, S or G₂/M and analysed by western blotting and probed for the presence of PKC-ßII. Blots were reprobed with an anti-lamin B antibody to confirm equal loading of gels. (B) The densitometric ratio of PKC-:lamin B immunoreactive bands was calculated for cells in G₁, S and G₂/M. (C) Cells in G₁, S or G₂/M were indirectly immunostained for PKC-ßII using a FITC-conjugated secondary antibody (green fluorescence) and were counterstained with propidium iodide to locate the nucleus (red fluorescence). Immunofluorescence was visualised using a confocal microscope. The data shown in A and C are representative of three separate experiments, and for B the data are mean±s.d. of three separate experiments; ^*P<0.02.