Fig. 4. Activation of classical PKC isoenzymes by SAG in vitro. Recombinant human PKC-, ßII and were combined with SAG in micelle form and PKC activation measured in an in vitro assay by incorporation of [³²P]ATP into histone H1 in the presence of calcium and PS. The results are presented as pmol of ³²P incorporated per minute per mg PKC and are the mean of triplicates for a single experiment, representative of three separate experiments.