Fig. 3. The relative mRNA distribution of the class V myosins in northern and dot blots shows that Myo5a is most abundantly expressed in brain, whereas Myo5b and Myo5c have a broad distribution. Myo5c is particularly abundant in secretory or epithelial tissues. Human mRNA blots (Clontech) were hybridized to probes for Myo5a, Myo5c and Myo5c. Subsequent incubations with control probes (ubiquitin or ß-actin) revealed that the mRNA loadings were in fact well normalized (not shown).