Fig. 6. Overexpressing the GFP-Myo5c tail leads to the formation of puncta and tubulovesicular structures. In transfected cells, the GFP-Myo5c tail forms puncta and tubules (A,B,D,G), suggestive of a membranous compartment, whereas GFP alone has a diffuse distribution (C). (D-F) Antibodies to Myo5c stain the puncta. (G-I) The puncta formed by overexpression of the GFP-Myo5c tail do not colocalize with endogenous Myo5a, which shows a striking localization to small puncta in the periphery of the cell.