Fig. 7. The GFP-Myo5c tail does not colocalize with or perturb the Golgi compartment, late endosomes or lysosomes. Arrows denote cells expressing the GFP-Myo5c tail, whereas arrowheads mark untransfected cells. (A-C) The GFP-Myo5c tail does not colocalize with golgin97, a membrane marker for the Golgi compartment. (D-F) Although the mannose-6-phosphate receptor cycles between the TGN, late endosomes, and lysosomes, at steady state the majority of the receptor is found on late endosomes, where it does not colocalize with the GFP-Myo5c tail. (G-I) The GFP-Myo5c tail also fails to colocalize with LAMP1, a membrane marker for lysosomes.