Fig. 8. The overexpressed GFP-Myo5c tail colocalizes with and perturbs a compartment containing the transferrin receptor and rab8, but not rab11a. In all panels, arrows denote transfected cells and arrowheads denote untransfected cells. (A-C) The overexpressed GFP-Myo5c tail colocalizes with the transferrin receptor, a marker for the endosomal pathway. Furthermore, the distribution of the transferrin receptor is perturbed in cells expressing the GFP-Myo5c tail (arrows) as compared to untransfected cells (arrowheads). (D-I) Transfected cells were also stained for rab proteins to define specific membrane compartments. The GFP-Myo5c tail puncta colocalize with rab8 (D-F), but not rab11a (G-I). Similar to the results with the transferrin receptor, at least some of the rab8 distribution appears perturbed in transfected cells (arrow, F), whereas the distribution of rab11a does not appear to be significantly altered in transfected cells (arrows, I) versus untransfected cells (arrowhead, I).