Fig. 9. In cells expressing the GFP-Myo5c tail, transferrin is taken up normally and colocalizes with the Myo5c tail after a short delay, but transferrin trafficking is inhibited. Cells were incubated in TRITC-transferrin for 10 minutes, followed by incubation in unlabeled transferrin for various `chase' times of 0, 5, 20 or 60 minutes. (A-C) Cells expressing the GFP-Myo5c tail (arrows) appear to internalize transferrin similar to untransfected cells (arrowhead). After 10 minutes of internalization, transferrin does not significantly colocalize with the GFP-Myo5c tail puncta, but by 20-60 minutes (D-I), transferrin shows obvious colocalization with the GFP-Myo5c tail. Furthermore, transferrin remains in cells expressing the GFP-Myo5c tail (arrows) for much longer than in untransfected cells (arrowheads), indicating that the GFP-Myo5c tail impedes transferrin trafficking. (J-K) Close examination of cells after 5 minutes of chase, when transferrin first begins to colocalize with the GFP-Myo5c tail, reveals that transferrin appears to associate with the margins of the GFP-Myo5c tail puncta (arrows).