Fig. 5. Ulp1 protein levels vary in response to increased temperature. Strain sp.611 (ulp1-13myc) was grown overnight to mid-log phase at 30°C (A) or at 25°C (B). TCA extracts were analysed by SDS PAGE and western blotting using anti-myc antisera (upper panels) or anti-tubulin antisera (lower panels). (A) Effect of ionising radiation and HU. Lane 1, no treatment; lane 2, 20 mM HU for 3 hours; lane 3 cells exposed to 500 Gy ionising radiation and incubated for a further 1.5 hours at 30°C. (B) Effect of temperature. Lane 1, no treatment; lane 2, 3 hours at 30°C; lane 3, 3 hours at 35.5°C.