Fig. 6. Ulp1 protein is located at the nuclear periphery in a cell-cycle-dependent manner. S. pombe strain sp.611 containing an integrated myc epitope-tagged version of ulp1 was used for DAPI staining and immunofluorescence with anti-myc antisera (TRITC) (A,B) and anti-tubulin antisera (FITC) (B). (A) Field of cells from an asynchronously growing culture. Cells, from the top to the bottom of the field, are in late M/G1, S, early anaphase and G2. (B) Composite of cells taken from an asynchronous culture, arranged in sequence according to cell cycle stage as determined by anti-tubulin staining. Merge is an overlay of DAPI and anti-myc antisera images. A number of examples of each stage are shown to give the range of intensity of Ulp1 staining.