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Fig. 10. Confocal analysis of GFP-Rac constructs targeting to vacuolar and lumenal membranes. (A) GFP-Racwt and (B) GFP-Rac1V12 localize to vacuolar EC membranes (arrowheads) as well as lumenal membranes (open arrows). Vacuolar fusion events (arrows) appear to occur between vacuoles and the lumenal membrane. Serial sections are 1 µm thick. Bar=20 µm.