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Fig. 11. Confocal analysis of GFP-Cdc42wt localization to EC vacuolar membranes and lumenal structures. (A) Targeting of GFP-Cdc42wt to vacuolar (arrowheads) and lumenal membranes (arrows). (B) Three-dimensional reconstruction of a multicellular structure containing a lumen (L). Left: propidium iodide (PI) staining of EC cultures indicating multiple cells interconnect to form a lumenal compartment. Nuclei are indicated with arrowheads. Right: GFP-Cdc42wt (shown in red) localizes to lumenal membranes (arrows).