JCS -- Nelson et al. 115 (6): 1137 Figure IG3

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Fig. 3. Combined imaging of p65-dsRed localisation and I ${kappa}$ B ${alpha}$ -EGFP degradation following TNF ${alpha}$ stimulation of dual-transfected cells. (A) Quantification of I ${kappa}$ B ${alpha}$ -EGFP degradation in dual-transfected cells also expressing either p65-dsRed or the control expression vector pdsRedN1. I ${kappa}$ B ${alpha}$ -EGFP fluorescence was determined as described in Fig. 2. (B) Quantification of translocation of p65-dsRed in dual-transfected cells also expressing either I ${kappa}$ B ${alpha}$ -EGFP or the control EGFP-N1. The nuclear: cytoplasmic ratio of p65-dsRed fluorescence was determined as described in Fig. 1. Results show mean values±s.d._(n-1) from two experiments, using at least four cells from each experiment.