Fig. 5. Long term NF-B response to TNF. (A) Long term widefield fluorescence microscopy of p65-localisation in response to TNF. Cells were treated at t=0 hours with 10 ng/ml TNF or carrier (control). Mean nuclear fluorescence intensities were determined for each cell at each time point. Results show mean values obtained from two experiments. (B) Transcriptional regulation of NF-B-sensitive promoter. Luciferase expression monitored in living cells transfected with pNF-B-luc. Cells were transfected in 24-well microtitre plates. 1 mM luciferin was added to cells 12 hours prior to addition of TNF (10 ng/ml final concentration) or carrier added at t=0 hours [n=4, results show means±s.d._(n-1)].