Fig. 7. Quantification of differential accumulation of p65-dsRed and IB-EGFP in the nucleus following treatment of dual-transfected cells with leptomycin B. (A) Cells transfected with p65-dsRed and IB-EGFP were treated for 2 hours with 10 ng/ml LMB and monitored by confocal microscopy. (B) Confocal microscopy of long term LMB-treated cells. The top panel was treated for 8 hours with 10 ng/ml LMB. The bottom panel was incubated with 10 ng/ml LMB followed 1 hour later by stimulation with 10 ng/ml TNF. The cells were then analysed for a further 8 hours following TNF stimulation.