JCS -- Nelson et al. 115 (6): 1137 Figure IG8

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 8. Quantitative analysis of p65 translocation, I ${kappa}$ B ${alpha}$ degradation and NF- ${kappa}$ B-dependent transcription in single living cells. (A) Dual confocal microscopy images of p65-dsRed and I ${kappa}$ B ${alpha}$ -EGFP at different time intervals following 1 hour LMB treatment followed by TNF ${alpha}$ stimulation. Images are shown before and after the 1 hour LMB treatment and 40 minutes after TNF ${alpha}$ stimulation. (B) Quantitative analysis of the time course of p65-dsRed translocation, I ${kappa}$ B ${alpha}$ -EGFP degradation and cell luminescence (luciferase activity) in three cells a-c (A,C) as described in Fig. 6. (C) Luminescence images of cells following TNF ${alpha}$ stimulation. Luminescence directed by NF- ${kappa}$ B-promoter-directed luciferase activity. Cells marked a-c correspond to those marked in A and used for quantification in B.