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Fig. 3. PC-GFP transport complexes do not label for ERGIC-53 or COPI during transport to the Golgi. Cells expressing PC-GFP in the presence of ascorbic acid were fixed 10 minutes after the addition of ascorbate and processed for immunofluorescence with antibodies directed against COPI (B) or ERGIC-53 (D). Note that PC-FP structures (A) do not label for COPI (B) and similarly PC-GFP TCs (C) do not label for ERGIC-53 (D, arrows). Bars=5 µm.