Fig. 4. PC-GFP transport complexes do not label for ts-045-G during transport to the Golgi. Those that do label for COPI contain both PC-FP and ts-045-G. Cells expressing PC-FP and ts-045-G-FP were incubated for 16 hours at 39.5°C, after which ascorbate and cycloheximide were added to the culture medium for 30 minutes. Cells were then incubated for 6 minutes at 32°C, fixed with paraformaldehyde and processed for immunofluorescence. PC-CFP TCs (A,D) largely exclude ts-045-G (B,E; arrow) and COPI (C,F). (D-F) An enlargement of the corresponding regions in (A-C), respectively. Those TCs that do label for both PC-CFP (A,D; arrowhead) and ts-045-G-YFP (B,E; arrowhead) also label for COPI (C,F; arrowhead). In contrast, TCs containing ts-045-G-YFP (G; arrowhead) also contain ERGIC-53 (H; arrowhead) and COPI (I; arrowhead). Bars=5 µm.