Fig. 8. Inhibition of COPI function by microinjection of anti-EAGE results in colocalisation of PC-FP with ERGIC-53. (A) and (B) show control cells expressing PC-FP (A) to which ascorbate was added 30 minutes before microinjection of control IgG. After a further 30 minutes at 37°C, cells were fixed and immunolabelled with anti-ERGIC-53 (B). Note structures containing PC-FP in (A; arrows) that do not label for ERGIC-53 in (B; arrows). (C,D) show cells expressing PC-FP (C) that, 30 minutes after the addition of ascorbate, were microinjected for anti-EAGE, incubated for a further 30 minutes at 37°C, fixed and immunolabelled for anti-ERGIC-53 (D). Note structures containing PC-FP in (C; arrowheads) that also label for ERGIC-53 in (D; arrowheads). The insets in (C) and (D) show zoomed areas from the highlighted regions. Bar=5 µm (applies to all panels).