Fig. 3. Colocalization of CD151 with the laminin receptor 7ß1 in smooth and skeletal muscle. Cryostat sections of human smooth and striated muscle were processed for double immunofluorescence confocal microscopy using P48 against CD151 (A) and polyclonal antibodies against 7B (B). The composite image (C) was generated by superimposition of the green and red signals, with areas of overlap appearing as yellow. The distribution pattern of CD151 and 7B completely overlap both at the sarcolemma of striated skeletal muscle (lower half) and on smooth muscle cells present in vessel walls (upper half). Note the prominent presence of CD151 in endothelial cells and the small amounts of CD151 in the stroma. Bar, 10 µm.