Fig. 5. CD151 is strongly associated with both splice variants (X1 and X2) of 7Bß1 in transfected K562 cells. K562 cells stably expressing the 7ß1 extracellular splice variant X1 or X2 were lysed in 1% CHAPS (C) or 1% Nonidet P-40 (N). Lysates were subjected to immunoprecipitation with mAb P48 against CD151 (upper panel) or with polyclonal antibodies against 7B (lower panel). The presence of 7B or CD151 in the immune complexes was detected by immunoblotting with anti-7B or anti-CD151 (mAb 8C3) antibodies. No immunoreactive bands corresponding to 7B were detected in control (ctrl) immunoprecipitates (upper panel). As a further control (ctrl), the presence of CD151 in total lysates is shown (lower panel). Note that 7B is coprecipitated with CD151 and vice versa, and that this is independent of the extracellular splice variant or the lysis buffer used.