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Fig. 7. Monoclonal antibody TS151R does not recognize CD151 when it is bound to {alpha}3ß1 or {alpha}7ß1. Transfected K562 cells stably expressing {alpha}3ß1 (left), {alpha}7ß1 (middle) or {alpha}Vß1 (right) were lysed in 1% Nonidet P-40 and subjected to immunoprecipitation with the mAbs P48 or TS151R, followed by immunoblotting with the indicated polyclonal antibodies against {alpha}3, {alpha}7 or {alpha}V. Immunoblot analysis of the lysates (LY) show the level of expression of each integrin.