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Fig. 1. MMH-D3 cells display a polarized phenotype and respond to the growth inhibitory function of TGF-ß1. (A) Phase contrast and confocal immunofluorescence microscopy of parental MMH-D3 cells stained with the adherens junction markers E-cadherin and ß-catenin and the tight junction marker ZO-1. (B) Proliferation kinetics of MMH-D3 cells (circles) versus MMH-D3 supplemented with 5 ng/ml TGF-ß1 (squares). (C) Flow cytometry determining the cell cycle distribution of MMH-D3 cells versus MMH-D3 at day 5 of TGF-ß1 (5 ng/ml) treatment.