Fig. 6. Molecular characteristics of epithelial MMH-R versus established fibroblastoid MMH-RT cells. (A) TGF-ß1 production of epithelial and fibroblastoid cell types. The amount of latent TGF-ß1 secretion into the medium was determined by ELISA. (B) Semi-quantitative RT-PCR determining the selective decrease (left panel) and increase (right panel) of mRNA abundance in epithelial versus fibroblastoid cells. All samples contained equal amounts of cDNA. As a control, rhoA mRNA expression remained unaffected (right panel). Lane 1, epithelial MMH-R cells; lane 2, fibroblastoid MMH-RT cells.