Fig. 7. Reversion of fibroblastoid MMH-RT cells to an epithelium-like phenotype. (A) Phase contrast microscopy of MMH-RT cells (control) grown on tissue culture plates and either treated with 30 µM UO126 or 5 µM LY294.002 for 24 hours. (B) Confocal immunofluorescence microscopy of MMH-RT cells treated with 5 µM LY294.002 for 24 hours. (C) Expression of E-cadherin and MMP-9 as determined by immunoblotting. Staining of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is shown as a loading control. Lane 1, fibroblastoid MMH-RT cells; lanes 2 and 3, MMH-RT cells treated with 5 µM LY294.002 for 24 and 72 hours, respectively; lane 4, epithelial MMH-R cells.