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Fig. 2. {alpha}1-PDX expression inhibits 3T3-L1 adipocyte differentiation. (A) Three control and 16 {alpha}1-PDX 3T3-L1 independent transfectant cell lines at confluence were treated (or not) with adipogenesis-inducing agents for 2 days. They were subsequently cultured for 5 days, fixed and stained with Oil Red O on day 7. A typical staining of differentiated and undifferentiated cells is shown. Unlike control cells, {alpha}1-PDX transfectant cell lines failed to respond to the adipogenic treatment. (B) Expression of {alpha}1-PDX transgene by RT-PCR in control and {alpha}1-PDX transfectants cell lines. Transcripts were observed in {alpha}1-PDX cells and not in control cells.