Fig. 2. Dual labeling of the FN matrix with FN-yfp and the actin cytoskeleton with cfp-Moe. Cells were cultured for 48 hours to allow matrix assembly and stained with polyclonal antibody for FN (c) or with phalloidin for actin (e). (a, b and d) show the fluorescence of yfp and cfp as indicated, and in (f), the yfp and cfp images are superimposed. Bar, 50 µm.