Fig. 3. Western blot of FN-yfp. Conditioned media from a 3-day confluent culture was analyzed by SDS-PAGE (5%) under reducing condition followed by western blotting with polyclonal antibodies for FN (a) and gfp (b). (a) shows that non-transfected 3T3 cells express native FN and FN-yfp/cfp-Moe transfected cells express both FN-yfp and native FN. (b) shows that the transfected 3T3 cells secrete about half as much FN-yfp as the FN-gfp secreted by the transfected CHO cells.