Fig. 4. Cellular protrusions induced by N. meningitidis are associated with ezrin recruitment and actin polymerization. Monolayers of HUVECs were infected for 4 hours with the ROU strain of N. meningitidis. A,B,C: xy sections performed by confocal microscopy of double fluorescence labeling ((A) bacteria and F-actin) or triple fluorescence labeling ((B) bacteria, F-actin and paxillin staining; (C) F-actin, ezrin and CD44) in the same fields. (D) xz section of a double fluorescence labeling of F-actin and ezrin. (A,C,D): x200; (B) x63.