Fig. 4. Localization of DmUba2 during embryogenesis. (A-I) Localization of DmUba2 in syncytial-blastoderm embryos during nuclear cycle 10 (A-C), 11 (D-F), and 13 (G-I). Surface views of embryos in interphase (A1-A3, D1-D3, G1, and G2) or mitosis (A4, D4, and G3) are shown; optical sections parallel to the surface and through the middle of nuclei in interphase (B1-B3, E1-E3, H1, and H2) or mitosis (B4, E4, and H3) and medial longitudinal optical sections of embryos in interphase (C1-C3, F1-F3, I1, and I2) or mitosis (C4, F4, and I3) are also shown. Each vertical set of three images (e.g., A1, B1, and C1) shows the same embryo. Each row of interphase images (e.g., A1, A2, and A3) is ordered according to the presumed sequence of the embryos within interphase, with the earliest point on the left (e.g., A1), based on the assumption that DmUba2 must gradually reaccumulate in the nucleus after its dispersion during mitosis. (J,K) Localization of DmUba2 in older embryos in stage 9 (J) or 12 (K). Medial longitudinal optical sections are shown. Arrow indicates a mitotic domain. Bar, 10 µm (A-I); 50 µm (J and K).