Fig. 6. Localization of DmUba2 and DmSmt3 at other developmental stages and in other cell types. (A-C) Localization of DmUba2 to nuclei during oogenesis. Egg chambers were double labeled with anti-DmUba2 and anti-Pnut. Arrows and arrowheads indicate structures discussed in the text. (A) Ovariole with the germarium on the left and successively older egg chambers moving left to right. DmUba2 (top panel; green in bottom panel) and Pnut (middle panel; red in bottom panel) are shown. (B) Overexposure of DmUba2 staining of an ovariole similar to that shown in A. (C) Higher magnification of a germarium, showing enrichment of DmUba2 (green) in nuclei of both follicle and germ cells, as well as in the nuclei of the muscle cells in the sheath surrounding the germarium. (D,E) Embryonic central nervous system after double staining for Pnut (red) and either DmUba2 (green in D) or DmSmt3 (green in E). (F,G) DmSmt3 localization to nuclei of syncytial blastoderm embryos during interphase (G) and to the chromosomes during mitosis (F). (H) Localization of DmSmt3 (green) to interphase nuclei during embryogenesis. Embryos at stages 8 (H1), 10 (H2), and 14 (H3) are shown.