Fig. 1. PMA-induced secretion of protein and immunoreactive cortical granule lectin from Xenopus oocytes. Groups of 75 stage II or single stage VI oocytes were incubated without or with PMA (1 µM) as indicated. After 30 minutes, the fluid surrounding the oocytes was collected for analysis by SDS-polyacrylamide gel and Coomassie staining or by western blot using antibodies against cgl. Samples were treated with DTT, as indicated. Mobility of molecular weight standards is indicated in kDa.