Fig. 2. Subcellular distribution of cortical granule lectin immunoreactivity before and after PMA-induced secretion. Single stage VI oocytes were incubated without or with PMA (1 µM) for 30 minutes as indicated. After recovering the fluid surrounding each oocyte (Rel), the oocytes were fractionated (see Materials and Methods) to generate supernatant (Sup) and pellet (Pel) fractions for immunoblot analysis using cgl antibody. Samples were not reduced with DTT (see Fig. 1). The +PMA example shown here reflects the most extensive release of cgl immunoreactivity observed in six trials.