Fig. 3. Association of endosomal/granule markers and cytosolic proteins with the MCPv, MCPa and SCP. Neutropils (4x10⁸ cells) were allowed to ingest H37Rv, H37Ra or S. aureus for 60 minutes. Thereafter, the phagosomes were isolated and equal amounts of the protein associated with the phagosomes were assayed for hck, lactoferrin and LAMP-1 and for annexin I, III and V by western blotting with appropriate antibodies. The illustrated immunoblots are representative of five independent experiments.