Fig. 10. Cholesterol depletion affects the cellular distribution of EGFR. Untreated HEp-2 cells (control) or HEp-2 cells preincubated with 10 mM MßCD for 15 minutes (MßCD), 1 ug/ml U18666A for 48 hours (U18666A), or 0.4 mg/ml water-soluble cholesterol for 30 minutes (cholesterol), were biotinylated as described in Materials and Methods. The fraction of biotinylated EGFR (plasma-membrane-localized EGFR) was calculated. Biotinylated EGFR in control cells was set to be 1. The figure shows pooled data from three independent experiments (means±s.e.m.).