Fig. 7. Comparison of FRAP experiments for the MAN1-GFP fusion protein (see Fig. 6) in the ER and nuclear envelope membranes. Fluorescence intensities in recovery after photobleaching are plotted versus time for the fusion protein in the ER (x) and nuclear envelope (o). Data points were obtained at 2 second intervals. The curves displayed kinetics allowing for the determination of diffusion constants for MAN1-GFP in the ER and nuclear envelope. Fluorescence intensity was normalized to prebleach intensity and corrected for total loss of fluorescence because of the high-energy laser bleach to I₀=100 (normalized prebleach intensity).